Pathogen susceptibility data

Spreadsheet "Susceptibility" contains the results of the pathogen susceptibility assay. The aphids used in this experiment either contained no known secondary symbionts or were artificially infected with different complements of symbiont species. Groups of 20 aphids, representing one of five genotypes and hosting one of seven symbiont complements, were exposed to spores of the pathogenic fungus Pandora neoaphidis. After six days all aphids were scored as a) sporulating; b) dead but not sporulating; or c) alive. In the spreadsheet, each row represents a replicate with 20 aphids. Columns are as follows: >>>Species - Aphid species, two levels (A. pisum, S. avenae). >>>Gen - Aphid genotype, five levels (145, J68, J102, Co23, Co26) >>>Sym - Symbiont infection status. Ha / Ri / Sp represent Hamiltonella defensa, Rickettsia sp. or Spiroplasma sp., and the value after underscore represents the genotype of original pea aphid host. >>>Fung_D6 - The number of aphids in a replicate which were classified as sporulating six days after the exposure. >>>Dead_D6 - The number of aphids in a replicate which were classified as dead but not sporulating. >>>Alive_D6 - The number of aphids in a replicate which were alive six days after the exposure. >>>Total - The total number of aphids in the three previous categories. Note that in some cases the total is different than 20 – occasionally not all aphids were found six days after the exposure, or the number of the exposed aphids in a replicate may have been miscalculated by 1 or 2.电子表格"Susceptibility"存储了病原菌敏感性测定（pathogen susceptibility assay）实验的结果。本实验所用的蚜虫，要么未携带任何已报道的次级共生菌（secondary symbionts），要么经人工接种了不同组合的共生菌物种。每组包含20头蚜虫，分别对应5种蚜虫基因型之一，并携带7种共生菌组合中的一种，将这些蚜虫暴露于病原真菌新蚜虫疠霉（Pandora neoaphidis）的孢子中。接种6天后，对所有蚜虫进行三类评分：a）产孢；b）死亡但未产孢；c）存活。该电子表格中，每一行代表一组含20头蚜虫的生物学重复（replicate）。各列含义如下： >>>Species（蚜虫物种）：共2个水平，分别为豌豆蚜（A. pisum）、麦长管蚜（S. avenae）。 >>>Gen（蚜虫基因型）：共5个水平，分别为145、J68、J102、Co23、Co26。 >>>Sym（共生菌感染状态）：Ha、Ri、Sp分别代表防御汉密尔顿菌（Hamiltonella defensa）、立克次体属物种（Rickettsia sp.）以及螺原体属物种（Spiroplasma sp.）；下划线后的数值代表原始宿主豌豆蚜的基因型。 >>>Fung_D6（产孢蚜虫数）：该生物学重复中，暴露处理6天后被判定为产孢的蚜虫数量。 >>>Dead_D6（死亡未产孢蚜虫数）：该生物学重复中，暴露处理6天后被判定为死亡但未产孢的蚜虫数量。 >>>Alive_D6（存活蚜虫数）：该生物学重复中，暴露处理6天后仍存活的蚜虫数量。 >>>Total（总蚜虫数）：上述三类蚜虫的总数。需注意，部分样本的总数量并非20——偶尔会出现6天后未能找回全部蚜虫的情况，或单个重复中初始接种蚜虫数的统计误差为1至2头。