Homologs of the genes lanJA, sprF1, sprF2, and sprOR are found associated with both known lanthipeptide BGCs and close to the sprPT/sprH3 gene pair.

Homology was determined at a cutoff of 30% amino acid identity of the gene products. Within Streptomyces genomes, all homologs were found within the analyzed 1,295 genomes. It was then checked whether these homologs overlapped with an antiSMASH-detected lanthipeptide BGC or were within 15 genes of the sprPT/sprH3 gene pair. sprOR homologs were found within canonical lanthipeptide BGCs as well as associated with the spriPT/sprH3 gene pair, suggesting its association with lanthipeptide BGCs. For non-Streptomyces genomes, the sprPT/sprH3 gene pair was first detected, and homologs of the given queries were found within the 15 surrounding genes. Homologs of lanJA and sprF1 are often found associated with sprPT/sprH3 gene pair, suggesting they are involved in lanthipeptide biosynthesis. (XLSX)