Antimutagenic and antirecombinagenic activities of noni fruit juice in somatic cells of Drosophila melanogaster

Noni, a Hawaiian name for the fruit of Morinda citrifolia L., is a traditional medicinal plant from Polynesia widely used for the treatment of many diseases including arthritis, diabetes, asthma, hypertension and cancer. Here, a commercial noni juice (TNJ) was evaluated for its protective activities against the lesions induced by mitomycin C (MMC) and doxorrubicin (DXR) using the Somatic Mutation and Recombination Test (SMART) in Drosophila melanogaster. Three-day-old larvae, trans-heterozygous for two genetic markers (mwh and flr3 ), were co-treated with TNJ plus MMC or DXR. We have observed a reduction in genotoxic effects of MMC and DXR caused by the juice. TNJ provoked a marked decrease in all kinds of MMC- and DXR-induced mutant spots, mainly due to its antirecombinagenic activity. The TNJ protective effects were concentration-dependent, indicating a dose-response correlation, that can be attributed to a powerful antioxidant and/or free radical scavenger ability of TNJ.

诺丽果（Noni，夏威夷语对巴戟天（Morinda citrifolia L.）果实的称谓）是源自波利尼西亚的传统药用植物，被广泛应用于多种疾病的治疗，涵盖关节炎、糖尿病、哮喘、高血压及癌症。本研究以商业诺丽果汁（TNJ）为研究对象，借助黑腹果蝇（Drosophila melanogaster）的体细胞突变和重组试验（Somatic Mutation and Recombination Test, SMART），评估其对丝裂霉素C（mitomycin C, MMC）与多柔比星（doxorubicin, DXR，原文存在拼写笔误）诱导的机体损伤的保护活性。实验选取携带mwh与flr3两个遗传标记的反式杂合3日龄幼虫，将其分别与TNJ联合MMC、TNJ联合DXR进行共处理。结果显示，该果汁可减轻MMC与DXR引发的遗传毒性效应：TNJ可显著降低MMC及DXR诱导的各类突变斑点数量，其保护作用主要源于抗重组活性。此外，TNJ的保护效果呈浓度依赖性，存在明确的剂量-反应相关性，这一特性可归因于TNJ强大的抗氧化能力与/或自由基清除能力。