RNA-seq and ATAC-seq analysis on mesenchymal stem cells

Mesenchymal stem cells (MSCs) are known to be heterogeneous; MSCs from different tissues show characteristic phenotypes. However, underlying molecular mechanisms of this MSC heterogeneity have not been clarified yet. In this study, to comprehensively understand transcriptome and epigenome of MSCs, we have analyzed multiple MSCs by RNA-seq and ATAC-seq. Mesenchymal stem cells (MSCs) were isolated from bone marrow (femur, vertebra), lung, and adipose tissue. Same procedure was repeated three times to prepare three lines for each tissue type MSC. RNA-seq and ATAC-seq were conducted in triplicates using standard protocols.