Barley seed developmental series

To provide comprehensive spatiotemporal information about biological processes in developing grains of cultivated barley (Hordeum vulgare subsp. vulgare), we performed a transcriptomic study of the embryo, endosperm, and seed maternal tissues collected from 4 to 32 days after pollination. Three main seed tissues (embryo, endosperm, and seed maternal tissues) were manually dissected at five developmental time points 4, 8, 16, 24 and 32 days after pollination (DAP). 4 DAP embryo and 32 DAP seed maternal tissues samples were omitted due to their minute size and dry nature, respectively. The total RNA was extracted, processed into the polyA-enriched libraries, and sequenced on the Illumina platform.