Supporting data for "the Prenylated Rab Acceptor PRA1.F3 acts in vacuolar protein transport in Arabidopsis thaliana"

The dataset include the plasmid sequence, the picture of the protein localization and the picture of GUS staining. The plasmid sequence include the plasmid used for  transient system, coimmunoprecipitation and yeast two-hybrid assay.
the protein localization include the localization in stable and transient system.The protein expression in seedling was examined using GUS staining assay, and the picture included in the dataset.
    The dataset/research is used to testify the localization and function of PRA1 protein in membrane system and find the interactor of PRA1 by which the molecular mechanisms of PRA1 in plant was unraveled that is meaniful for understanding the vesicle trafficking.

本数据集包含质粒序列、蛋白质定位图像以及GUS染色图像。其中，质粒序列涵盖了用于瞬时系统、共免疫沉淀和酵母双杂交实验的质粒。蛋白质定位图像展示了在稳定和瞬时系统中的定位情况。通过GUS染色实验对幼苗中的蛋白质表达进行了检测，相关图像亦包含在数据集中。本数据集/研究旨在验证PRA1蛋白在膜系统中的定位和功能，并通过寻找与PRA1相互作用的蛋白，揭示PRA1在植物分子机制中的功能，这对于理解囊泡转运具有重要意义。