Survivability and developmental ability of vitrified embryos stored in different EFS solutions at –80°C for 160 days.

*The embryos transferred were different from those used for in vitro culture experiments.
**Surviving 2-cell embryos were transferred into the oviducts of pseudopregnant recipient females.
Numbers with different superscripts within the same column are significantly different (P<0.05 by Chi-squared test with Yates’ correction).