UTX condensation underlies its tumor suppressive activity

We investigated whether UTX condensates regulate local H3K4me1 in the genome by ChIP-seq of THP-1 cells expressing empty vector (control), UTX WT, ΔcIDR, UTX-eIFIDR, or ΔTPR. To determine the effect of UTX condensation on high-order chromatin interactions or looping, we performed H3K4me3 HiChIP in duplicates to generate high-resolution contact maps around the active and poised transcription start sites (TSSs) in THP-1 cells expressing empty vector (control), UTX WT, ΔcIDR, or UTX-eIFIDR. ChIP was performed and libraries were constructed and sequenced with 20 million THP-1 cells expressing empty vector (control), UTX WT, ΔcIDR, UTX-eIFIDR, or ΔTPR. HiChIP experiments were performed with 2.5 million THP-1 cells expressing empty vector (control), UTX WT, ΔcIDR, UTX-eIFIDR, or ΔTPR using the Arima-HiC+ kit according to its recommended protocol.