Anne Carpenter (2011) CIL:21788, Drosophila melanogaster, epithelial. CIL. Dataset

Five different samples of Drosophila melanogaster Kc167 cells were stained with Hoechst 33342, a DNA stain. The last sample (labeled nodsRNA, CIL 21789) is of wild-type cells. Each of the other four samples (labeled 48, 340, Anillin, and mad2, CIL 21749, 21739, 21759, and 21773, respectively) has a different gene knocked down by RNAi. This is the set that were treated with mad2 RNAi. There are 10 images of each sample, for a total of 50 images. The images were acquired on a Zeiss Axiovert 200M microscope. A recommended use for these five image sets is to algorithmically compute the number of cells in each image. A tab-delimited text file containing this information can be found at the URL indicated in the attribution box. The recommended citation when using these images is: "We used the Drosophila Kc167 1 image set (Carpenter, et al., Genome Biology, 2006) available from the Broad Bioimage Benchmark Collection (www.broad.mit.edu/bbbc)."

本数据集包含五个不同品系的果蝇黑腹果蝇Kc167细胞的样本，经Hoechst 33342染料进行DNA染色。其中，最后一份样本（标记为nodsRNA，编号CIL 21789）为野生型细胞。其余四个样本（分别标记为48、340、Anillin和mad2，编号依次为CIL 21749、21739、21759和21773）均通过RNA干扰技术敲除不同的基因。该数据集特指经mad2 RNA干扰处理的样本集。每个样本包含10张图像，总计50张图像。这些图像是在蔡司Axiovert 200M显微镜下采集的。建议利用这些图像集进行算法计算每张图像中的细胞数量。包含该信息的制表符分隔的文本文件可从所附归属框中的URL获取。在使用这些图像时，建议引用如下：‘我们使用了源自Broad生物图像基准集合（www.broad.mit.edu/bbbc）的果蝇Kc167 1图像集（Carpenter等，基因组生物学，2006年）’。