Gfap-CT2;tdTOM_Baghdadi et al.2021

Intestinal swiss rolls of serial sections from Gfap-creERT2;R26tdTomato mice. Recombination efficiency is assessed by detection of tdTomato+ cells using RFP antibody two weeks post tamoxifen treatment (n=9 mice/genotype; ≥500 crypts/n). Ecadherin staining is used to mark epithelial cells. A representative intestinal swiss rolls show no glial cell maker gene expression in the epithelium.来自Gfap-creERT2；R26tdTomato双转基因小鼠的连续切片小肠卷包标本（intestinal swiss rolls）。于他莫昔芬处理两周后，通过红色荧光蛋白（RFP）抗体检测tdTomato阳性细胞以评估重组效率，每组基因型包含9只小鼠，每个样本至少统计500个肠隐窝。采用上皮钙粘蛋白（E-cadherin）染色标记上皮细胞。一份代表性的小肠卷包标本显示，上皮细胞中未检测到神经胶质细胞标记基因的表达。