The transgenerational effects of mitochondrial supplementation

To determine the outcomes of offspring produced from mitochondrial supplementation to mature oocytes at the time of fertilisation, we generated three generations of offspring. Primordial follicles of the third generation female mice (postnatal day 3) were collected, and RNA sequencing was performed. We compared the treated and control gene expression data to determine differentially expressed genes between the two groups. Our data suggest that mitochondrial supplementation has transgenerational effects on offspring. Overall design: 1. mitochondrial supplementation at the time of fertilisation was performed 2. three generations of offspring were produced to determine the transgeneration effects of the technique. 3. Primordial follicles of the third generation day 3 postnatal offspring were collected and extracted to compare the transcripts of treated vs control primordial follicles.