Characterizing human pluripotent stem cell derived kidney organoids by single-cell RNA sequencing of whole organoids and bulk RNA sequencing of organoid-derived podocytes for comparison with immortalized podocytes and in vivo podocytes

We performed single-cell sequencing to characterize the cell types that are present in human induced pluripotent stem cell derived (iPS) kidney organoids and their transcriptional profile. Furthermore, using bulk RNA sequencing we compared the transcriptional profile of kidney organoid derived podocytes from a wildtype iPS line, an iPS line with 2 mutations in podocin (NPHS2) which causes clinical manifestation of nephrotic syndrome and an iPS line with one of these mutations repaired which causes no clinical symptoms. For reference and comparison we took along human in vivo glomeruli and a human conditionally immortalized podocyte cell line. For single-cell RNA sequencing, we used one organoid sample (no replicates). For bulk RNA sequencing, we included three samples per condition (N=3, except for wildtype 2 were we had an n=2) of the following conditions: Mutant, repaired, wildtype, wildtype2, glomerulus, ciPOD. **RAW DATA not provided due to patient privacy concerns**