Development of a 3D co-culture model to generate porcine mammary epithelial cell spheroids

This data set contains results related to 3D culture protocol to generate mammary multicellular spheroids using Göttingen Minipigs primary cells: mammary epithelial cells (mpMECs), aortic endothelial cells (mpAECs), and vascular-wall mesenchymal stem cells (mpVW-MSCs). Two cellular culture methods were applied: hanging drop (HD) and ultra-low attachment plate (ULA).
Brightfield area, eccentricity, viability and cell distribution were analysed.
The results described the formation of irregular shaped aggregates when cultured by hanging drop (HD) (see data file ConcePTION_mpMECs-co-colture_HD_20250723.xlsx), while more compact and viable spheroids were formed by ultra-low attachment plate (ULA) method (see data files ConcePTION_Spheroid-Area_ULA_20250723.xlsx, ConcePTION_Spheroid-Eccentricity_ULA_20250723.xlsx and ConcePTION_Viability_ULA_20250723.xlsx).