Crystal structure of ASO binding Fab fragment with ASO143

Crystal structure of ASO binding Fab fragment with ASO143 Descriptor: 1,2-ETHANEDIOL, 1-[(1~{S},3~{R},4~{R},7~{S})-7-[[(1~{R},3~{R},4~{R},7~{S})-7-[[(1~{R},3~{R},4~{R},7~{S})-3-(6-aminopurin-9-yl)-7-[[(1~{R},3~{R},4~{R},7~{S})-3-(4-azanyl-5-methyl-2-oxidanylidene-pyrimidin-1-yl)-7-oxidanyl-2,5-dioxabicyclo[2.2.1]heptan-1-yl]methoxy-sulfanyl-phosphoryl]oxy-2,5-dioxabicyclo[2.2.1]heptan-1-yl]methoxy-sulfanyl-phosphoryl]oxy-3-(4-azanyl-5-methyl-2-oxidanylidene-pyrimidin-1-yl)-2,5-dioxabicyclo[2.2.1]heptan-1-yl]methoxy-sulfanyl-phosphoryl]oxy-1-(hydroxymethyl)-2,5-dioxabicyclo[2.2.1]heptan-3-yl]-5-methyl-pyrimidine-2,4-dione, 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID, ... Authors: Hsia, H.-E, Zanini, C, Simonneau, C, Fraidling, J, Kraft, T, Mayer, K, Sommer, A, Indlekofer, A, Wirht, T, Benz, J, Georges, G, Langer, L.M, Gassner, C, Larraillet, V, Manso, M, Ravn, J, Hofer, K, Emrich, T, Niewoehner, J, Schumacher, F, Brinkmann, U. Deposit date: 2024-08-09 Release date: 2025-08-20 Last modified: 2025-11-12 Method: X-RAY DIFFRACTION (1.48 Å) Cite: Improved targeted delivery of antisense oligonucleotide with an antibody mask. Nucleic Acids Res., 53, 2025反义寡核苷酸（ASO，antisense oligonucleotide）结合抗原结合片段（Fab，Fragment Antigen Binding）与ASO143的晶体结构 描述组分：1,2-乙二醇（1,2-ETHANEDIOL）、1-[(1S,3R,4R,7S)-7-[[(1R,3R,4R,7S)-7-[[(1R,3R,4R,7S)-3-(6-氨基嘌呤-9-基)-7-[[(1R,3R,4R,7S)-3-(4-氨基-5-甲基-2-氧代嘧啶-1-基)-7-羟基-2,5-二氧杂双环[2.2.1]庚烷-1-基]甲氧基(硫代)磷酰]氧基]-2,5-二氧杂双环[2.2.1]庚烷-1-基]甲氧基(硫代)磷酰]氧基-3-(4-氨基-5-甲基-2-氧代嘧啶-1-基)-2,5-二氧杂双环[2.2.1]庚烷-1-基]甲氧基(硫代)磷酰]氧基-1-(羟甲基)-2,5-二氧杂双环[2.2.1]庚烷-3-基]-5-甲基嘧啶-2,4-二酮、4-(2-羟乙基)-1-哌嗪乙磺酸（4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID）等。作者：Hsia, H.-E、Zanini, C、Simonneau, C、Fraidling, J、Kraft, T、Mayer, K、Sommer, A、Indlekofer, A、Wirht, T、Benz, J、Georges, G、Langer, L.M、Gassner, C、Larraillet, V、Manso, M、Ravn, J、Hofer, K、Emrich, T、Niewoehner, J、Schumacher, F、Brinkmann, U。提交日期：2024-08-09；发布日期：2025-08-20；最后更新日期：2025-11-12。实验方法：X射线衍射（X-RAY DIFFRACTION，分辨率1.48 Å）。引用文献：《抗体掩蔽策略优化反义寡核苷酸的靶向递送》（Improved targeted delivery of antisense oligonucleotide with an antibody mask），《核酸研究》（Nucleic Acids Res.），第53卷，2025年。