Seasonal changes of gene expression of brains (ventral part of telenchephalon, hypothalamus and pituitary) in Medaka fish (Oryzias latipes)

To examine the seasonal adaptaion, we compared the gene expression of brains (ventral part of telenchephalon, hypothalamus and pituitary) between SD (short-day conditions: 10 h light/14 h dark and 26 °C) and LD (long-day conditions: 14 h light/10 h dark and 26 °C) conditions in Medaka fish (Oryzias latipes). The gene expression of brains (ventral part of telenchephalon, hypothalamus and pituitary) was measured during the transition from SD to LD conditions. For each time point, cRNAs prepared from pooled brains (ventral part of telenchephalon, hypothalamus and pituitary) (n=2) were hybridized to duplicate sets of microarrays.