Data from: Mapping of two suppressors of OVATE (sov) loci in tomato

Tomato fruit shape varies significantly in the cultivated germplasm. To a large extent, this variation can be explained by four genes including OVATE. While most varieties with the OVATE mutation bear elongated fruits, some accessions carry round fruit suggesting the existence of suppressors of OVATE in the germplasm. We developed three intraspecific F_2 populations with parents that carried the OVATE mutation but differed in fruit shape. We used a Bulk Segregant Analysis approach and genotyped the extreme classes using a high-throughput genotyping platform, the SolCAP Infinium Assay. The analyses revealed segregation at two QTL, sov1 and sov2. These loci were confirmed by genotyping and QTL analyses of the entire population. More precise location of those loci using progeny testing confirmed that sov1 on chromosome 10 controlled obovoid and elongated shape whereas sov2 on chromosome 11 controlled mainly elongated fruit shape. Both loci were located in intervals of less than 2.4 Mb on their respective chromosomes.栽培番茄种质资源的果实形状变异极为丰富。该性状的变异在很大程度上可由包括OVATE基因在内的四个基因解释。尽管携带OVATE突变的多数品种果实呈长形，但部分种质材料的果实为圆形，这表明该种质资源中存在OVATE基因的抑制因子。本研究构建了三个种内F₂群体，其亲本均携带OVATE突变，但果实形状存在差异。研究采用集群分离分析法（Bulk Segregant Analysis），借助高通量基因分型平台SolCAP Infinium Assay对极端表型类群进行基因分型。分析结果显示存在两个分离的数量性状基因座（Quantitative Trait Locus, QTL），分别为sov1与sov2。通过对全群体进行基因分型与QTL分析，验证了这两个基因座的存在。后续通过后代测验对这两个基因座进行精确定位后证实：10号染色体上的sov1可调控倒卵形与长形果实形状，而11号染色体上的sov2主要调控长形果实形状。两个基因座在各自染色体上的定位区间均小于2.4 Mb。