Additional file 16: of Anopheline salivary protein genes and gene families: an evolutionary overview after the whole genome sequence of sixteen Anopheles species

Divergence of orthologous salivary proteins among anophelines and in the subgenera Cellia, Anopheles and Nyssorhynchus. (Worksheet 1_salivary genes divergence) Protein name, corresponding An. gambiae accession number, size in amino acids, location on the An. gambiae chromosomes and average percent aminoacid identity ± standard deviation are reported. N, number of sequences; S, number of variable sites; d, number of amino acid substitution per site (average over all sequence pairs); dS, number of synonimous substitutions per synonimous site; dN, number of nonsynonimous substitutions per nonsynonimous site; Pi, nucleotide diversity per site; SE, standard error; SD, standard deviation. Average identities ± standard deviations were calculated for each protein from the pairwise comparisons between the different species. Only An. gambiae s.s. was included in the analysis as representative of the An. gambiae species complex. The grey shading indicates absence of the gene in the corresponding subgenus. NA, not applicable. Salivary proteins with percentage of identity below 50% (orange), between 50 and 60% (green), 60 and 70% (yellow) and above 90% (light blue) are highlighted. Identities in 12 orthologous housekeeping proteins are shown for comparison. EIF1A, Eukaryotic Initiation Factor 1A; G6PD, glucose-6-phosphate 1-dehydrogenase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; GSTT1, glutathione S-transferase theta class 1; rpL5, ribosomal protein L5; rpS7, ribosomal protein S7; SDH, succinate dehydrogenase. (Worksheet 2_sorted by dN/dS) Salivary protein genes were sorted by dN/dS ratio. The thirteen with the higher dN/dS ratio and including all the seven SG1 family members are boxed. (XLSX 94 kb)