Overexpression of RBFOX1 in HEK293 cells and the effect on circRNA formation

We carried out two mammalian transcriptomics experiments to compare the effect of overexpressing the splicing factor RBFOX1 on circRNA formation. We include quantification of gene expression and circRNA expression. Overall design: Each of the experiments used HEK293 cells and comprised six samples, 3 control replicates and 3 RBFOX1 over-expression replicates. Sequencing was performed on both ends of the cDNA fragment (paired-end reads).