Matlab script for quantification of presynaptic terminal anatomical features.

Length of terminal membrane, active zone, and glial facing membrane compartments are measured, along with point coordinates of all presynaptic vesicles. These values are used to calculate the Euclidean distance between the centre point of each vesicle and the nearest pixel of presynaptic terminal membrane, active zone and glial facing membrane. Fraction of total terminal length covered by glia, and midpoint distance between presynaptic terminal and glial membrane are also calculated. Script imports coordinate data from manually-labelled Image J file and exports table of distance measurements and outline image of terminal compartments and vesicles. (M)