A comprehensive gene expression analysis to identify the key regulator suppressing the IFN-γ-inducible factors in tumor cells

Immune checkpoint inhibitors (ICIs) provide clinical benefits for various advanced malignancies. However, the predictive factors that determine sensitivity to ICIs have not been fully elucidated. To identify the mechanisms underlying ICI resistance, we performed a microarray analysis to compare the IFN-γ-inducible genes between ICI-sensitive AB1-HA and ICI-resistant LLC in vitro. Total RNA was extracted from AB1-HA or LLC tumor cells incubated with or without IFN-γ for 24 h.