RNA-sequencing of splenic RNA from mice with compound mutations in ADAR1 and ZBP1
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The purpose of this study was to examine the role of ZBP1 in the phenotype that develops when ADAR1 activity is missing, in particular when the Zalpha domain of ADAR1 is mutated. Mice homozygous for a Zalpha domain-mutant allele of Adar1 (Adar1mZalpha/mZalpha mice) were compared with control mice carrying one mZalpha allele and one wild type allele of Adar1 (Adar1wt/mZalpha mice) and with mice carrying one mZalpha and one null Adar1 allele (Adar1-/mZalpha mice). Adar1-/mZalpha mice were also compared with mice additionally deficient in ZBP1 (Adar1-/mZalpha Zbp1-/- mice). Given the early postnatal lethal phenotype that develops in Adar1-/mZalpha mice, comparisons were made in RNA isolated from spleen tissue from newborn mice of each genotype (5 mice per genotype).
