Data on genetic diversity of circumsporozoite protein (csp) non-repeat regions from Plasmodium knowlesi clinical isolates of Sabah.

This dataset presents an analysis in the genetic diversity of malaria circumsporozoite protein (csp) of Plasmodium knowlesi in Sabah; where circumsporozoite protein is one of the targeted candidates for malaria vaccine development and was conducted to evaluate the suitability of csp as a vaccine in relation to its genetic diversity. The data were collected from 26 human blood spot samples from Kudat and Kota Kinabalu hospitals in Sabah in 2012 which were tested positive for malaria.

Genomic DNA extraction, nested PCR, cloning and sequencing of the csp genes were carried out and phylogenetic, sequence diversity and natural selection of the csp genes were analysed using bioinformatic tools such as MEGAX and DnaSP ver. 5.10.00 for phylogenetic tree build, mutational analysis and neutral theory of evolution. Analysis and comparison of this gene was done against P. knowlesi csp strain H as a reference sequence (GenBank database XM_002258966.1) showed point mutations at 52 positions among the 237 sequences. The phylogenetic tree revealed that the occurrence of multiple haplotypes was scattered despite of geographical location. The evolutionary history which was inferred using the Neighbor-Joining method (Saitou and Nei, 1987) revealed no geographical clustering to any country listed above; with a total of 76 non-repeat region Pkcsp haplotypes including one haplotype unique to this study (haplotype H12).
These data could serve as auxiliary information and/or research data for other researchers in Sabah. It could also serve as guide or reference data to other researchers outside Sabah who may be interested in carrying out similar research in other states.

本数据集对沙巴地区恶性疟原虫（Plasmodium knowlesi）子孢子蛋白（circumsporozoite protein，简称csp）的遗传多样性进行了分析；子孢子蛋白是疟疾疫苗研发的潜在靶点之一，本研究旨在评估csp作为疫苗的适宜性及其遗传多样性。数据来源于2012年沙巴库达特和哥打基纳巴卢医院采集的26份人类血液斑点样本，这些样本经检测确认感染疟疾。
基因组的DNA提取、csp基因的巢式PCR扩增、克隆和测序工作已完成，并利用MEGAX和DnaSP ver. 5.10.00等生物信息学工具对csp基因的进化树、序列多样性和自然选择进行了分析。与恶性疟原虫csp菌株H（GenBank数据库XM_002258966.1）作为参考序列进行基因分析和比较显示，在237个序列中存在52个位置上的点突变。进化树分析表明，尽管地理位置各异，多个单倍型的发生却呈散布状态。通过邻接法（Saitou and Nei, 1987）推断的进化历史揭示了无任何国家存在地理聚类；总计包含76个非重复区域Pkcsp单倍型，其中包含一个在本研究中独特的单倍型H12。
这些数据可为沙巴地区其他研究人员提供辅助信息和研究数据，同时也可作为指南或参考数据，供其他对在其他州进行类似研究感兴趣的研究人员参考。