NONO preferentially binds to exon-intron boundary and regulates alternative splicing in Rat Cardiac Fibroblasts [RIP-seq]

Myocardial fibrosis occurs in a variety of cardiac diseases. As one of the markers of cardiac activated-myofibroblast protein, NONO may be involved in this process while the underlying mechanisms have not been explored. NONO, assumed to hold a role in the regulation of global transcriptome profile in cardiac fibrotic, was silenced in rat cardiomyocytes. RNA-seq and improved RNA immunoprecipitation and sequencing (iRIP-seq) were used to analyze the dysregulated transcriptome by NONO, as well as the NONO-bound RNAs in the control and siNONO group. Quantitative PCR and western blotting were applied to determine NONO-regulated mRNA and protein expression levels in rat cardiomyocytes. RNA-seq and iRIP-seq were successfully performed, and NONO-bound RNAs were characterized. Transcrptional profile of rat cardiac fibroblasts cells with/without GENE_NONO Knockdown were generated