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Materials for "Neuron ID dataset facilitates neuronal annotation for whole-brain activity imaging of C. elegans"

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Mendeley Data2024-06-29 更新2024-06-29 收录
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https://figshare.com/articles/Materials_for_Neuron_ID_dataset_facilitates_neuronal_annotation_for_whole-brain_activity_imaging_of_C_elegans_/8341088/1
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This dataset is materials for a paper entitled "Neuron ID dataset facilitates neuronal annotation for whole-brain activity imaging of C. elegans"(Preprint on bioRxiv: https://www.biorxiv.org/content/10.1101/698241v2) This dataset includes following materials: Additional file 1: Dataset S1 (zip): Neuron ID dataset (contains positions and expression patterns) and corresponding static 3D images.Additional file 2: Table S1 (xlsx): Summary of neuron ID dataset including expression patterns of the promoters. Additional file 3: Figure S1 (pdf): Correction of posture of the worms.Additional file 4: Figure S2 (pdf): Performance and robustness of the posture correction.Additional file 5: Table S2 (xlsx): Summary statistics for Additional file 4: Figure S2. Additional file 6: Figure S3 (pdf): Movements of the cells during time-lapse imaging.Additional file 7: Table S3 (xlsx): Summary statistics for Additional file 6: Figure S3. Additional file 8: Figure S4 (pdf): Overlay plot of cell positions for all worms Additional file 9: Figure S5 (pdf): Specific-cell-centered landscape. Additional file 10: Figure S6 (pdf): Less varying neuron pairs.Additional file 11: Figure S7 (pdf): Position of posterior pharyngeal bulb affects cell positionsAdditional file 12: Table S4 (xlsx): Summary statistics for Additional file 11: Figure S7.Additional file 13: Figure S8 (pdf): Stability and sparseness of expression pattern.Additional file 14: Table S5 (xlsx): Evaluation result of promoter combinations.Additional file 15: Figure S9 (pdf): An example fluorescent image of JN3039 strain and annotated cell names.Additional file 16 (zip): Dataset S2: Positions of nuclei and expression patterns of landmark fluorescence in the whole-brain imaging strains as the test data for automatic annotation and corresponding static 3D images.Additional file 17: Figure S10 (pdf): Health of 4D strains.Additional file 18: Table S6 (xlsx): Summary statistics for Additional file 17: Figure S10.Additional file 19: Figure S11 (pdf): Comparison of the synthetic atlas and the neuron ID datasetAdditional file 20: Figure S12 (pdf): Variation of relative position of cell pairsAdditional file 21: Figure S13 (pdf): Error rate of each bipartite matching and majority voting.Additional file 22: Figure S14 (pdf): Relationship between error rate of automatic annotation for JN3039 and detected count in the neuron ID datasetAdditional file 23: Figure S15 (pdf): Error rates of the automatic annotation method for the animals in a microfluidic chip Additional file 24: Dataset S3 (zip): A tutorial for semi-automatic annotation using our software.Additional file 25: Figure S16 (pdf): Correct ratio of automatic annotation and its improvement by manual annotationAdditional file 26: Figure S17 (pdf): Most valuable cells for improving accuracy of automatic annotation Additional file 27: Note S1 (docx): Optimization of parameters for atlas generationAdditional file 28: Dataset S4 (zip): Sequences of the promoters in Table 1.Additional file 29: Dataset S5 (zip): All codes for the GUI RoiEdit3D and analysis pipeline to make figures.

本数据集为一篇题为《神经元ID数据集助力秀丽隐杆线虫(C. elegans)全脑活动成像的神经元注释》的论文配套材料(预印本发布于bioRxiv:https://www.biorxiv.org/content/10.1101/698241v2)。本数据集包含以下配套材料: 附加文件1:数据集S1(压缩包格式):神经元ID数据集(包含神经元位置与表达模式)及配套静态三维图像。 附加文件2:表格S1(xlsx格式):神经元ID数据集汇总,包含启动子的表达模式信息。 附加文件3:图S1(pdf格式):线虫姿态校正方法。 附加文件4:图S2(pdf格式):姿态校正的性能与鲁棒性。 附加文件5:表格S2(xlsx格式):附加文件4(图S2)的汇总统计数据。 附加文件6:图S3(pdf格式):延时成像过程中的细胞运动情况。 附加文件7:表格S3(xlsx格式):附加文件6(图S3)的汇总统计数据。 附加文件8:图S4(pdf格式):所有线虫的细胞位置叠加图。 附加文件9:图S5(pdf格式):以特定细胞为中心的可视化图谱。 附加文件10:图S6(pdf格式):变异程度较低的神经元对。 附加文件11:图S7(pdf格式):咽部后球位置对细胞位置的影响。 附加文件12:表格S4(xlsx格式):附加文件11(图S7)的汇总统计数据。 附加文件13:图S8(pdf格式):表达模式的稳定性与稀疏性。 附加文件14:表格S5(xlsx格式):启动子组合的评估结果。 附加文件15:图S9(pdf格式):JN3039菌株的荧光成像示例及标注后的神经元名称。 附加文件16(压缩包格式):数据集S2:全脑成像菌株的细胞核位置与标志性荧光表达模式,作为自动注释的测试数据,及配套静态三维图像。 附加文件17:图S10(pdf格式):四维成像菌株的健康状况。 附加文件18:表格S6(xlsx格式):附加文件17(图S10)的汇总统计数据。 附加文件19:图S11(pdf格式):合成图谱与神经元ID数据集的对比。 附加文件20:图S12(pdf格式):神经元对的相对位置变异情况。 附加文件21:图S13(pdf格式):各二分匹配与多数投票的错误率。 附加文件22:图S14(pdf格式):JN3039菌株自动注释错误率与神经元ID数据集的检测计数之间的相关性。 附加文件23:图S15(pdf格式):微流控芯片中线虫的自动注释方法错误率。 附加文件24:数据集S3(压缩包格式):使用本团队开发软件进行半自动化注释的教程。 附加文件25:图S16(pdf格式):自动注释的正确率及人工注释带来的准确率提升。 附加文件26:图S17(pdf格式):提升自动注释准确率的关键神经元。 附加文件27:补充说明S1(docx格式):图谱生成的参数优化方法。 附加文件28:数据集S4(压缩包格式):表格1中提及的启动子序列。 附加文件29:数据集S5(压缩包格式):GUI工具RoiEdit3D的全部源码及绘图所用的分析流程代码。
创建时间:
2023-06-28
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