FAK activity alters the transcriptional landscape during SMC phenotypic switching

Purpose: The goals of this study are to compare the effect of FAK activity on gene expression of NGS-derived RNA-sequencing during vascular remodeling. Overall design: Methods: Mice were treated with or without FAK-I for 2 weeks after wire injury. Femoral arteries were isolated, and the fat and adventitia layers were carefully removed under surgical microscope. To remove endothelial and immune cells, the artery lumen was quickly flushed with 150 µl TRIzol lysis reagent (Invitrogen) using a 29-gauge insulin syringe. Media were frozen in liquid nitrogen and lysed in 500 µl TRIzol per artery, and total RNA was isolated using the RNeasy column. Isolated RNA submitted for NGS library generation and sequencing. All experiments performed in triplicate.