Identification of GRF9 early responding genes using an estradiol-inducible overexpression (XVE) system.

The aim of the experiment was to identify genes rapidly responding at their expression level to enhanced expression of the transcription factor GRF9. The name of the transgenic plant used is GER 9-13. The goal was to identify genes rapidly (within a few hours) responding to enhanced expression of growth regulating factor 9 (GRF9) in Arabidopsis thaliana. To this end, 2-week-old seedlings (2 samples) and detached mature leaves (from 40-day-old soil grown plants - one sample) were induced by 10 µM estradiol for 3, 4 or 6 h respectively. Expression profilling was performed using Affymetrix ATH1 microarrays. Mock-treated seedlings (0.1% ethanol) were used as controls.