Table S1 - On the Molecular Basis of D-Bifunctional Protein Deficiency Type III
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Changed rare amino acid codons in HsMFE-2 cDNA. In total 13 codons for arginine and 2 for isoleucine that are rare for E. coli were replaced with more common ones without changing the original amino acid sequence to increase the expression level of the recombinant HsMFE-2 protein. The point mutations were done by using a QuikChangeTM site-directed mutagenesis kit (Stratagene). (DOC)
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2015-12-02
