A dataset of clinical parameters of infertile women undergoing IVF, including FSHB c.-211G>T and FSHR c.2039A>G genotypes

A dataset of clinical parameters of infertile women undergoing through ovarian stimulation for assisted reproductive technologies in Rostov-on-Don, Russia. A short gonadotropin-releasing hormone antagonist protocol was used for ovarian stimulation. Early-follicular-phase follicle-stimulating hormone, luteinizing hormone, anti-mullerian hormone and progesterone levels were assayed in serum on 3-5-th menstrual cycle day 5-6 cycles before ovarian stimulation, with “Access hFSH”, “Access hLH”, “Access AMH” and “Access Progesterone” kits using Automatic Immunochemical Analyzer “Access 2” (Beckman Coulter, USA). Genotyping of FSHB c.-211G>T [rs10835638] genetic variant was performed using restriction fragment length polymorphism (RFLP) analysis with restriction endonuclease TatI and primers: 5´- GGAGCCAGATCATGAAATGTT-3´, 5´- GACCAATGCTAGCCTGAAGC-3´. Genotyping of FSHR c.2039A>G [rs6166] genetic variant was performed using RFLP analysis with restriction endonuclease BsrI and primers: 5´-TTTGTGGTCATCTGTGGCTGC-3´, 5´-CAAAGGCAAGGACTGAATTATCATT-3´. The PCR products detection was performed by horizontal electrophoresis in 2% agarose gel. All samples were validated by double genotyping.

本数据集收录了俄罗斯顿河畔罗斯托夫地区接受辅助生殖技术卵巢刺激治疗的不孕女性临床参数。卵巢刺激采用短效促性腺激素释放激素（gonadotropin-releasing hormone）拮抗剂方案。于卵巢刺激前5~6个月经周期的第3~5天采集血清样本，使用美国贝克曼库尔特（Beckman Coulter）公司的Access 2全自动免疫化学分析仪，搭配“Access hFSH”、“Access hLH”、“Access AMH”及“Access Progesterone”检测试剂盒，检测血清中的早卵泡期促卵泡生成素（follicle-stimulating hormone）、黄体生成素（luteinizing hormone）、抗穆勒氏管激素（anti-mullerian hormone）及孕酮水平。针对FSHB基因c.-211G>T [rs10835638]遗传变异的基因分型，采用限制性片段长度多态性（restriction fragment length polymorphism, RFLP）分析，使用限制性内切酶TatI及引物序列：5´- GGAGCCAGATCATGAAATGTT-3´、5´- GACCAATGCTAGCCTGAAGC-3´。针对FSHR基因c.2039A>G [rs6166]遗传变异的基因分型，同样采用RFLP分析，使用限制性内切酶BsrI及引物序列：5´-TTTGTGGTCATCTGTGGCTGC-3´、5´-CAAAGGCAAGGACTGAATTATCATT-3´。PCR产物的检测通过2%琼脂糖凝胶水平电泳完成。所有样本均通过双基因分型进行验证。