(16s) No 1. DADA2 Workflow (16S rRNA) Output

Output files from the No 1. DADA2 Workflow page of the Bocas Hypoxia study. File names and descriptions: **RUN01_read_changes.txt** : Tracking changes in read counts (per sample) from the beginning to end of the DADA2 workflow. **RUN02_read_changes.txt** : Tracking changes in read counts (per sample) from the beginning to end of the DADA2 workflow. **combo_pipeline.rdata:** contains sequence and taxonomy tables from the DADA2 pipeline needed for subsequent analyses. To see the **Objects** , in R run _load("combo_pipeline.rdata", verbose=TRUE) _ 1) seqtab.1: Sequence table from Run01 before merging with Run02. 2) seqtab.1: Sequence table from Run02 before merging with Run01. 3) st.sum: merged sequence table before removing chimeras 4) st.all: duplicate of st.sum 5) seqtab: merged sequence table after removing chimeras 6) tax_silva: Silva (v132) taxonomy table of seqtab 7) tax_gg: GreenGenes taxonomy table of seqtab本数据集为博卡斯缺氧研究（Bocas Hypoxia study）的DADA2分析流程第1号页面所产出的输出文件。 文件命名及说明如下： **RUN01_read_changes.txt**：用于追踪DADA2分析流程全程中，每个样本的读段（read）计数变化情况。 **RUN02_read_changes.txt**：用于追踪DADA2分析流程全程中，每个样本的读段（read）计数变化情况。 **combo_pipeline.rdata**：包含后续分析所需的DADA2分析流程产出的序列表与分类学表。若需查看其中包含的对象（Objects），可在R语言环境中执行代码`load("combo_pipeline.rdata", verbose=TRUE)`。 1) `seqtab.1`：Run01与Run02合并前生成的序列表。 2) `seqtab.1`：Run02与Run01合并前生成的序列表。 3) `st.sum`：去除嵌合体前的合并序列表。 4) `st.all`：`st.sum`的副本。 5) `seqtab`：去除嵌合体后的合并序列表。 6) `tax_silva`：对应`seqtab`的Silva（v132）分类学表。 7) `tax_gg`：对应`seqtab`的GreenGenes分类学表。