Comparative Proteomic Analysis Reveals Candidate Pathways Related to the Effect of Different Light Qualities on the Development of Mycelium and Fruiting Body of Pleurotus ostreatus

Light affects the morphology and
physiology of Pleurotus
ostreatus. However, the underlying molecular mechanism
of this effect remains unclear. In this study, a label-free comparative
proteomic analysis was conducted to investigate the global protein
expression profile of the mycelia and fruiting bodies of P. ostreatus PH11 growing under four different light
quality treatments. Among all the 2234 P. ostreatus proteins, 1349 were quantifiable under all tested conditions. A
total of 1100 differentially expressed proteins were identified by
comparing the light group data with those of the darkness group. GO
and KEGG enrichment analyses indicated that the oxidative phosphorylation,
proteasome, and mRNA surveillance pathways were the most related pathways
under the light condition. qRT-PCR verified that the expression of
the white collar 1 protein was significantly enhanced under white
light. Additionally, glutamine synthetase and aldehyde dehydrogenase
played important roles during light exposure. This study provides
valuable insight into the P. ostreatus light response mechanism, which will lay the foundation for improved
cultivation.