Analysis of TYK2 variants in cell lines over time and in different subclones.

Presence of the TYK2 R1027 and A35V variants was tested in the CCRF-CEM cell line from our group (“CCRF-CEM Cools lab”) as well as in the CCRF-CEM cell line as it is currently sold by DSMZ (“CCRF-CEM 2011 DSMZ (ACC240)) and in 5 different CCRF-CEM subclones that DSMZ collected over the years. Similarly, KARPAS-45 from the Cools lab and the KARPAS-45 lines obtained from DSMZ in 2011 and in 1994 were screened for presence of the TYK2 Q830* variant. JURKAT cells from the Cools lab as well as JURKAT provided by DSMZ in 2011 and 1992 were tested for the TYK2 C192Y variant.*This cell line has 4 copies of chromosome 19 containing TYK2. The height of the variant peak on the chromatogram suggests that only 1 copy of TYK2 contains the Q830* variant.