Online Supplementary of Shi et al manuscript June 21 2022.pdf

There are supplemental data and figures of one manuscript titled as VEGFR2 insufficiency enhances phosphotoxicity and undermines Klotho’s protection against peritubular capillary rarefaction and kidney fibrosis. This basic research manuscript is accepted for publication in American Journal of Physiology-Renal Physiology.

This research paper studied interplay of type 2 of vascular endothelial growth factor receptor (VEGFR2), high dietary phosphate and Klotho, an anti-aging protein, in peritubular structure and kidney fibrosis. Klotho protein was shown to maintain VEGFR2 expression in the kidney and reduce high phosphate-induced cell injury. But Klotho cytoprotection was attenuated by VEGFR2 inhibition. Thus, normal VEGFR2 function is required for vascular integrity and Klotho to exert vascular protective and anti-fibrotic actions in the kidney.