Spectrophotometric determination of LAP photoinitiator radicalization in ophthalmic applications

The incidence of lamellar corneal transplantations is increasing. While a human donor cornea could potentially be used for up to two recipients, one anterior and one posterior part, it does not change the disparity between available donor grafts and patients needing corneal transplants. Additionally, donor grafts carry the risk of graft rejection due to foreign cellular material and disease transmission. We have investigated acellular synthetic graft materials to eliminate these drawbacks using hydrogel materials and the extrusion bioprinting process. To create these materials with higher moduli, the photocrosslinking process has advantages but needs an appropriate crosslinker material. We have shown that we can determine the presence of the non-radicalized crosslinker Lithium Phenyl (2,4,6-Trimethylbenzoyl) Phosphinate (LAP) with spectrophotometric methods, using the crosslinker absorbance characteristics. To show non-radicalized LAP, the measurement is done at 372 nm, and the measured..., , # Spectrophotometric determination of LAP photoinitiator radicalization in ophthalmic applications

Dataset DOI: [10.5061/dryad.73n5tb38p](10.5061/dryad.73n5tb38p)

## Description of the data and file structure

Polymers were prepared and photocrosslinked with LAP. Optical, mechanical, and chemical testing were performed. All files are Microsoft Excel Worksheets. Samples are denoted as X-Y-Z where X is the methacrylated silk (SilMA) concentration, Y is the methacrylated gelatin (GelMA) concentration, and Z is the LAP crosslinker concentration. The Excel worksheets with \"Absorbance\" in the title are absorbance data for polymer samples with varying crosslinking times. The number in the title of these sheets is the wavelength. The \"Analysis of Crosslinking\" worksheet contains data from the plate reader with one sheet with plots. The three worksheets with \"TNBS\" in the title refer to the TNBS assays used to quantify the degree of methacrylation of the scaffolds. The data are the outputs fro...,