Supplementary figure 5

<b>Supplementary Figure 5: Effects of rapid E2 signaling on binge alcohol consumption, binge sucrose consumption, and avoidance behavior are dose- and ovarian E2 state-dependent (related to Fig. 4).</b> a-d) Behavioral effects of systemic estrogen synthesis inhibition using acute systemic administration of letrozole (LET) given 40 minutes prior to behavioral testing in high or low E2 status females. a) Acute LET (10 mg/kg i.p.) did not alter binge EtOH consumption in low E2 status females (N’s = 18 VEH, 20 LET. b) Acute systemic administration of a low dose of LET (1 mg/kg, i.p.) did not alter binge EtOH consumption in high E2 status females, while a high dose of LET did (Fig. 4; N’s = 10 VEH, 8 LET). c) Acute systemic LET (10 mg/kg, i.p) did not alter binge sucrose consumption in females in either a low ovarian E2 state (left, N’s = 13 VEH, 12 LET) or high ovarian E2 state (right, N’s = 9 VEH, 12 LET). d) Acute LET administration in high E2 status females 2 hours prior to EPM did not alter the % time spent in the open arms (left) or distance traveled (right; N’s = 9 VEH, 9 LET). e) BNST guide cannulae placements following histology (for Fig. 4f-i); each dot is an individual hit. Data are presented as mean values +/- SEM/ Detailed statistics are provided in Supplemental Table 1. Source data are provided as a Source Data file.