five

ATAC-seq analyses by using Tn5 transposome assembled with IEOE19 adaptor DNAs

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE144449
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To investigate the specificity of the Tn5 transposome with short adaptor DNAs under the non-crosslinked condition, we conducted ATAC-seq using alternative 19 bp adaptor DNAs (IE and OE) harboring 7 nucleotide differences between them (Maggie.JMB.1998), which allowed for specific PCR amplification without losing the superior enzymatic activity. ATAC-seq analyses by using Tn5 transposome assembled with different adaptor DNAs
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2022-05-02
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