Figure 5 Data

5a. Upregulated normalized gene expression from untreated patient with post-polycythemia-vera myelofibrosis. 5b. Cytokines of donor monocytes in stiff, viscous and elastic hydrogels +/-IPI-549. 5d. Mouse peripheral blood parameters eight weeks after transplantation with Jak2-V617F+ compared to wildtype cells. Spleen weight: measured five-to-seven months post-transplant. 5e. Nano-indentation of WT and transplant-recipient control or myelofibrotic femur BM. Mean storage modulus (and viscoelasticity (tan(delta)). 5f. Linear regression of tan(delta) with fraction of Jak2-V617F GFP+ cells in BM of recipient mice and myelofibrosis grading, as well as with storage modulus. 5g. Normalized fraction of CD11b+ and Ly6C+Ly6G- monocytes from empty-vector or Mx1-Cre-transplanted, non-fibrotic Mx1-Cre Jak2-V617F transplanted recipient controls and overtly fibrotic Jak2-V617F transplanted recipient BM. Values normalized to empty-vector or Mx1-Cre transplanted controls. 5h. Ly6C+Ly6G- monocytes and CD11c+ MHC-class-II I-A/I-E+ conventional DCs from fibrotic Jak2-V16F transplanted recipient BM treated daily p.o. for 2 weeks with vehicle control (-) or 15 mg/kg IPI-549 (+, 10% DMSO/5%NMP/85%PEG400).