Expression data from mice hematopoietic progenitor cells

Nfatc1 short isoform-specific KO mice are embryonic lethal. This isoform is essential for osteoclast differentiation and is responsible for the gene expression of various osteoclast markers, including the isoform itself. We used clariom s assay to explore genes showing altered expresssion during osteoclast differentiation in cultured hematopoietic progenitor cells from KO mice. To investigate the effect of RANKL and the Nfatc1 short isoform on gene expression profiles, probes for genomewide genes were hybridized with total RNA isolated from cultured cells. Four samples including hematopoetic prognitor cells from WT and KO mice treated with or without RANKL were used.