de novo transcriptomes of Goniopora columna, Galaxea astreata and Galaxea acrhelia

Transcriptomic resources for coral species can provide insight into coral evolutionary history and stress-response physiology. Goniopora columna, Galaxea astreata and Galaxea acrhelia are scleractinian corals of the Indo-Pacific, representing a diversity of morphologies and life-history traits. G. columna and G. astreata are common and cosmopolitan, while G. acrhelia is largely restricted to the coral triangle and Great Barrier Reef. Reference transcriptomes for these species were assembled from replicate colony fragments exposed to elevated (31°C) and ambient (27°C) temperature.Trinity was used to create de novo assemblies for each species from 92-102 million raw Illumina Hiseq 2 x 150 bp reads. Host-specific assemblies contained 65,460-72,405 contigs, representing 20,093-31,693 isogroups (~genes) with an average N50 of 2,254. Gene name and/or gene ontology annotations were possible for 61% of isogroups on average and transcriptomes contained 93.1-94.3% of KOGs comprising the core eukaryotic gene set, indicating fairly comprehensive assemblies.This work expands the complement of transcriptomic resources available for scleractinian coral species, including the first the first reference for a representative of Goniopora spp. as well as species with novel morphology. Data collected during these periods: 01 Aug 2016

珊瑚物种的转录组学资源，可为解析其演化历史与应激响应生理机制提供关键研究视角。柱形棘杯珊瑚（Goniopora columna）、星孔珊瑚（Galaxea astreata）及阿克雷利亚星孔珊瑚（Galaxea acrhelia）均为印太海域分布的石珊瑚（scleractinian corals），兼具多样的形态特征与生活史性状。其中柱形棘杯珊瑚与星孔珊瑚分布广泛且较为常见，而阿克雷利亚星孔珊瑚的分布范围则主要局限于珊瑚三角区（Coral Triangle）与大堡礁（Great Barrier Reef）。本研究针对上述三种珊瑚，以暴露于高温（31℃）与常温（27℃）环境的重复珊瑚群体片段为材料，组装获得其参考转录组。研究利用Trinity软件，对每个物种的9200万至1.02亿条原始Illumina HiSeq 2×150 bp测序读段进行从头组装。各物种的宿主特异性组装结果包含65460至72405条重叠群（contigs），对应20093至31693个同源基因簇（isogroups，即近似基因），平均N50值为2254。平均而言，61%的同源基因簇可获得基因名称及/或基因本体（gene ontology, GO）注释；所获转录组覆盖了核心真核基因集中93.1%~94.3%的KOG（真核直系同源蛋白簇），表明本次组装具有较高的完整性。本研究丰富了石珊瑚物种的转录组学资源储备，其中包含棘杯珊瑚属（Goniopora spp.）的首个参考转录组，以及具有新颖形态特征的星孔珊瑚物种参考转录组。本研究数据采集时间：2016年8月1日。