Transcriptomic Profiling on Kidneys of Mice after Cecal Ligation and Puncture (CLP) Compared to Sham Operation. Transcriptomic Profiling on Kidneys of Mice after Cecal Ligation and Puncture (CLP) Compared to Sham Operation

Sepsis is a severe clinical syndrome related to an exaggerated host immune response to infection as well as systematic inflammation and serious tissue damage. Sepsis-associated acute kidney injury (SA-AKI) is one of the most frequent and serious complications of sepsis. This study aimed to identify new mechanisms in SA-AKI through transcriptome profiling (RNA-seq). Overall design: A total of 5 C57BL/6 mice were randomly divided into 2 groups: CLP group (n=3), Sham group (n=2). In CLP group, we first anesthetized mice 2%～4% isoflurane. Under aseptic conditions, a 2-cm midline laparotomy was created below the diaphragm to expose the cecum. Two-thirds of the cecum were ligated with a 5-0 silk suture and punctured twice with a 22-gauge needle. The cecum was then squeezed gently to extrude a small amount of feces through the perforation site. Animals were resuscitated with 1 mL of saline subcutaneously after CLP. The procedures of the Sham group (controls) were the same as that of the CLP group, except for ligation and perforation. At 6 hours after CLP, the mice were sacrificed by neck fracture, and the kidneys were taken for RNA sequencing.