Next Generation Sequencing Analysis of Prenatal Chlorpyrifos Exposure embryonic kidneys

Purpose: In order to study signaling pathway changes of prenatal chlorpyrifos exposure embryonic kidney development Methods: By using RNA-seq, we studied the transcriptome of E12.5, E14.5, E16.5 and E18.5 prenatal chlorpyrifos exposure embryonic kidneys and DMSO exposure (control) embryonic kidneys Results: We show that Notch signaling pathway and aquaporin family had high expression in chlorpyrifos exposure E18.5 kidney. The nephron progenitor markers had low expression in chlorpyrifos exposure embryonic kidneys ICR mice aged 8 weeks were bred using timed matings, the pregnant mice were randomly divided into two groups. Chlorpyrifos (96% purity) was dissolved in dimethyl sulfoxide (DMSO), and CPF 5mg/kg was injected subcutaneously daily in CPF group during gestation day 7.5-11.5. The controls were injected with isovolumetric DMSO at the same time. We collected E12.5, E14.5, E16.5 and E18.5 mouse embryonic kidneys for RNA-seq of the two groups respectively.