<b>Genome assembly of the rare and endangered Grantham's camellia</b>, <b>Camellia granthamiana</b>

The Grantham’s camellia (Camellia granthamiana Sealy) is a rare and endangered tea species that is endemic to southern China, and was first discovered in Hong Kong in 1955. Despite its high conservation value, genomic resources of C. granthamiana remain limited. Here, we present a chromosome-scale draft genome of the tetraploid C. granthamiana (2n = 4x = 60) using a combination of PacBio long read sequencing and Omni-C data. The assembled genome size is ~2.4 Gb with most sequences anchored to 15 pseudochromosomes that resemble a monoploid genome. The genome is of high contiguity, with a scaffold N50 of 139.7 Mb, and high completeness with a 97.8% BUSCO score. Gene model prediction resulted in a total 68,032 protein-coding genes with a BUSCO score of 90.9%. 1.65 Gb of repeat content was annotated, which accounts for 68.48% of the genome. The Grantham’s camellia genome assembly provides a valuable resource for future investigations on its biology, ecology, phylogenomic relationships with other Camellia species, as well as set up a foundation for further conservation measures.<br>BioProject PRJNA973839 https://www.ncbi.nlm.nih.gov/bioproject/PRJNA973839<br>Assembly GCA_036172215 https://www.ncbi.nlm.nih.gov/datasets/genome/GCA_036172215.1

葛兰姆山茶（Camellia granthamiana Sealy）是一种珍稀濒危的茶属物种，为中国南方特有种，于1955年首次在香港被发现。尽管其具有极高的保护价值，但葛兰姆山茶的基因组学资源仍然十分有限。本研究结合PacBio长读长测序与Omni-C技术，报道了该四倍体葛兰姆山茶（2n = 4x = 60）的染色体级别草图基因组。组装后的基因组大小约为2.4 Gb，大部分序列被锚定至15条拟染色体，其结构与单倍体基因组相似。该基因组具有较高的连续性，支架N50（scaffold N50）为139.7 Mb，且完整性优异，BUSCO（Benchmarking Universal Single-Copy Orthologs）评分达97.8%。通过基因模型预测，共得到68032个蛋白质编码基因，对应的BUSCO评分为90.9%。本研究共注释得到1.65 Gb的重复序列，占基因组总长的68.48%。本研究获得的葛兰姆山茶基因组组装结果，为后续开展该物种的生物学、生态学、与其他山茶属物种的系统基因组学关系研究提供了宝贵资源，同时也为后续保护措施的制定奠定了基础。<br>BioProject PRJNA973839 https://www.ncbi.nlm.nih.gov/bioproject/PRJNA973839<br>Assembly GCA_036172215 https://www.ncbi.nlm.nih.gov/datasets/genome/GCA_036172215.1

搜集汇总

背景与挑战

背景概述

该数据集提供了稀有濒危植物Grantham's camellia（Camellia granthamiana）的染色体级别基因组组装，基于PacBio长读长测序和Omni-C技术构建，基因组大小约2.4 Gb，具有高连续性和完整性（scaffold N50为139.7 Mb，BUSCO评分97.8%），并包含68,032个预测的蛋白质编码基因。这些数据为研究该物种的生物学特性、生态学、系统发育关系以及保护措施提供了重要资源。