A single cohort study collecting interval timed incisional epidermal and dermal tissue samples during surgical procedures to profile temporal response of tissue after noxious stimuli

This study collects tissue from patients undergoing long surgeries at the NIH Clinical Center. Samples from these patients are collected from the wound edge at the initial incision, as well as 1 hours, 2 hours, 4 hours, 6 hours, and surgical closure (if the case lasts >=8 hours). These samples were subdivided in the operating room and processed for histology, RNA-Seq, and lipidomics. Patients were also evaluated for pain ratings pre-operatively and at post-operative days 1 and 2. These pain ratings included pain intensity and interference, as well as descriptors of patient pain modalities (using the McGill Questionnaire).