Manatee Springs Run 5: Vent Biofilm and Water Sampling

These data pertain to a cave dive in the cave system of Manatee Springs State Park, Levy County, Florida, USA made on October 29th 2025 with the aim of sampling a vent (seep) identified on the right hand wall of the main cave conduit approximately 1300ft upstream of Friedman's Sink. Six 50ml sterile syringes were used to collect water from inside the vent, a dark grey biofilm and underlying sediment was collected into sterile 50ml centrifuge tubes.. A 500ml nalgene bottle was used to collect water from the main conduit approximately 30ft upstream of the vent and samples of normal-appearing sediment were collected from the same location.