Investigation of the effect of Coriander (Coriandrum savitum) on hematological and immune system of Rainbow Trout (Oncorhynchus mykiss)

This study includes the investigation of the effect of coriander (Coriandrum savitum) on haematological parameters and immune system of rainbow trout (Oncorhynchus mykiss). For this purpose, fish with an average weight of 40±25 g were used. Coriander was added to the feed as 0, 0.5, 1.0, 2.0 mg/g, and experimental groups were formed. Prepared feed was fed in the morning and evening and weighed as 2% of fish weight daily. Blood samples were taken from the fish on the 21st and 42nd days from the first feeding. After the feeding experiment (21st and 42nd days), hematological and immunological parameters were examined. As a result, adding up to 2% of coriander to the feed of Rainbow trout did not cause any problems in terms of fish growth. In the study, it was observed that there was no difference between the control group and the experimental group in terms of erythrocyte, leukocyte, hemoglobin, hematocrit, average erythrocyte volume, mean erythrocyte hemoglobin, mean erythrocyte hemoglobin concentration, total immunoglubin, lysozyme activity, nitroblue tetrazolium activity, protein values, whereas in terms of myeloperoxidase activity (P >0.05), a difference was observed between the control group and the experimental group (P <0.05).

本研究围绕芫荽（Coriandrum savitum）对虹鳟（Oncorhynchus mykiss）血液学指标与免疫系统的影响展开探究。本次试验选取平均体重为40±25 g的虹鳟作为试验对象，将芫荽以0、0.5、1.0、2.0 mg/g的梯度比例添加至基础饲料中，以此设置实验组。每日早晚投喂制备完成的试验饲料，投喂量为试验鱼体重的2%。于首次投喂后的第21天和第42天采集试验鱼的血液样本，在投喂实验结束（即第21天与第42天）后，对血液学与免疫学相关指标进行检测。研究结果显示，在虹鳟饲料中添加最高达2.0 mg/g的芫荽，不会对鱼的生长造成任何不良影响。本研究观察到，对照组与各实验组在红细胞（erythrocyte）、白细胞（leukocyte）、血红蛋白（hemoglobin）、血细胞比容（hematocrit）、平均红细胞体积（average erythrocyte volume）、平均红细胞血红蛋白（mean erythrocyte hemoglobin）、平均红细胞血红蛋白浓度（mean erythrocyte hemoglobin concentration）、总免疫球蛋白（total immunoglubin）、溶菌酶活性（lysozyme activity）、氮蓝四唑活性（nitroblue tetrazolium activity）及蛋白含量等指标上均无统计学差异（P>0.05）；而在髓过氧化物酶活性（myeloperoxidase activity）方面，对照组与实验组间存在显著统计学差异（P<0.05）。