Essential Oil from Pinus Koraiensis pinecones Inhibits Gastric Cancer Cells via the HIPPO/YAP Signaling Pathway

The purpose of this study was to investigate the anti-tumor activity of PEO on MGC-803 cells and its mechanism. Anti-tumor experiments in vitro showed PEO could significantly inhibit the proliferation and migration of MGC-803 cells, and it also could arrest the cell cycle in G2/M phase, decrease the mitochondrial membrane potential and induce apoptosis. Finally, the effects of PEO on genes expression on MGC-803 cells were analyzed by RNA sequencing, and results showed that after treatment with PEO, 100 genes were up-regulated, and 57 genes were down-regulated. According to KEGG pathway and GSEA, FAT4, STK3, LATS2, YAP1 and AJUBA were down-regulated, which are related to HIPPO signaling pathway. Real-time PCR and western blot further confirmed the results of RNA sequencing. These results indicated that PEO may exert anti-tumor activity via the HIPPO/YAP signaling pathway. The RNA of MGC-803 cells treated and untreated were sequenced by high-throughput sequencing to detect the effect of drugs on the gene of MGC-803 cells. This research was funded by National Key R&D Program Project "Ecological Protection and Biological Resources Development and Utilization Technology and Demonstration in Northeast Forest Region" (sub-project) (No. 2016YFC0500305-02).