Transcriptome profiling of colorectal cancer cells on BZW2 knockdown.

Basic leucine zipper and W2 domains 2 (BZW2), also known as translation initiation regulator eIF5-mimic protein 1 (5MP1), is a member of the basic-region leucine zipper (bZIP) superfamily of transcription factors. BZW2 has partial homology with eIF5 and can function as a competitive inhibitor of eIF5. In human Colorectal cancers, high mRNA and protein expression levels of BZW2 were associated with tumor progression. BZW2-knockdown reduced malignant phenotypes, including cell proliferation, invasion, and spheroid and colony formation. BZW2-knockdown also reduced tumor growth and metastasis In summary, our study characterizes the expression BZW2 in colorectal cancer and explore its regulation mechanism. Future studies are needed to decipher the biological significance of these findings. Overall design: RNA from corectal cancer cell lines (HCT116, HT29 and SW480) treated with BZW2 shRNA and non-targeting shRNA were sequenced. RNA-seq experiment is intended to identify genes and pathways altered on BZW2 knockdown.