Phytophthora spp. identification, in vitro oomycide sensitivity trial, and in vivo root and collar diameter

The isolate sheet contains all the Phytophthora species isolates tested and identified as P. nicotianae or P. citrophthora using sequencing and PCR-RFLP. The isolate sheet shows the workflow to identify the Phytophthora spp. isolates. The in vitro trial data sheets show the P. nicotianae colony growth diameters measured in two separate trials of a dilution series of oomycide-amended media inoculated with Phytophthora nicotianae. SAS statistical analysis sheets calculate the EC50 values based on the measured colony growth diameters.
The in vivo trials evaluated the effect of ethaboxam and mandipropamid on root rot caused by Phytophthora nicotianae. The in vivo trial sheets contain the wet and dry root weight and collar diameter measurements taken at four harvest time points of a greenhouse trial. In the preventative in vivo trials, the oomycide was applied before P. nicotianae inoculation. The inverse is true for the curative in vivo trials. The oomycides applied to the in vivo trials were also ethaboxam and mandipropamid. The set also contains GenStat data which is analysed for statistical differences between different treatments.

该隔离表包含了所有经测序和PCR-RFLP鉴定为烟草疫霉（Phytophthora nicotianae）或柑橘疫霉（Phytophthora citrophthora）的疫霉物种分离物。隔离表展示了鉴定疫霉物种分离物的流程。体外试验数据表展示了在两个分别进行的稀释系列卵菌素（oomycide）改良培养基接种烟草疫霉的试验中测量的烟草疫霉菌落生长直径。SAS统计分析表基于测量的菌落生长直径计算EC50值。体内试验评估了乙硫甲威和吗啉膦对由烟草疫霉引起的根腐病的效应。体内试验表包含了在温室试验的四个收获时间点测量的湿根重和茎基直径。在预防性体内试验中，卵菌素在烟草疫霉接种前施用。相反，对于治疗性体内试验，情况则相反。用于体内试验的卵菌素同样为乙硫甲威和吗啉膦。该套件还包含了用于分析不同处理之间统计差异的GenStat数据。