Phytoremediation potential of organochlorine pesticide contaminated soils with Miscanthus x giganteus: comparing freshly spiked with legacy contaminated soils

This study compares the dissipation dynamics of DDT metabolites and HCH isomers. It evaluates the plant uptake ability and phytotoxicity of Miscanthus x giganteus (Mxg) in legacy contaminated soils (i.e., decades-long contamination) and freshly spiked soils.
DDT and its metabolites, as well as HCH isomers, demonstrated clear dissipation differences across both soils as a result of the age of the contaminant. The initial and final concentration of OCPs of interest show that Mxg did not have any role in the dissipation of the compounds. A root-to-soil ratio (R/S) (fold change) was calculated in order to assess the accumulation ability of the plant. HCHs resulted in a higher R/S ratio when compared to DDTs in both legacy contaminated and freshly spiked soil, and the uptake was isomer-selective. The phytotoxicity observed in this study has not been reported before.
The data given here is the concentration of DDT metabolites (for both o’chloro and p’chloro isomers) and HCH isomers (α-, β-, γ- and δ-) in the researched soil contamination and in the root system of the plant. A headspace solid-phase microextraction gas chromatography-mass spectrometry (HS–SPME–GC–MS) measurement was carried out. A Trace 1310 gas chromatograph (Thermo Fisher Scientific, San Jose, CA, USA), a CombiPAL autosampler (CTC Analytics AG, Zwingen, Switzerland) assembled with a SPME fibre assembly, and an ISQ 7000 mass spectrometer (Thermo Fisher Scientific) were used. The data was obtained in µg/L and then converted to µg/kg (Microsoft Excel, version 2504). About all performed calculations, treatments were considered as a single group.

本研究对比了滴滴涕（DDT）代谢物与六氯环己烷（HCH）异构体的消解动力学，评估了巨芒草（Miscanthus x giganteus, Mxg）在数十年污染历史的老旧污染土壤与新鲜投加污染土壤中的植物吸收能力及植物毒性。
由于污染物老化程度存在差异，滴滴涕及其代谢物、六氯环己烷异构体在两类土壤中均表现出显著的消解差异。目标有机氯农药（OCPs）的初始与终末浓度结果显示，巨芒草并未对目标化合物的消解过程产生影响。为评估该植物的富集能力，本研究计算了根土比（R/S，即倍数变化值）。在老旧污染土壤与新鲜投加污染土壤中，六氯环己烷的根土比均高于滴滴涕类化合物，且其吸收过程具有异构体选择性。本研究中观测到的植物毒性此前未见报道。
本研究提供的数据为目标土壤污染体系与植物根系中，滴滴涕代谢物（包括邻氯、对氯异构体）及六氯环己烷异构体（α-、β-、γ-及δ-型）的浓度。测试采用顶空固相微萃取-气相色谱-质谱联用法（HS–SPME–GC–MS）完成分析，所用仪器包括：美国加利福尼亚州圣何塞赛默飞世尔科技公司的Trace 1310气相色谱仪、配备固相微萃取纤维组件的瑞士茨温根CTC分析仪器公司CombiPAL自动进样器，以及赛默飞世尔科技公司的ISQ 7000质谱仪。原始数据以微克/升（µg/L）为单位，随后通过Microsoft Excel 2504版本转换为微克/千克（µg/kg）。本研究将所有开展的计算与实验处理步骤均视为单一实验组。