Dataset and R code for: Bacterial supplementation shapes honey bee gut microbiota and host metabolism under controlled and field conditions

This repository contains processed datasets, metadata, and scripts used to generate the figures and statistical analyses reported in the manuscript. Raw sequencing and proteomics data are deposited in public repositories and referenced in the manuscript. External repositories• 16S rRNA sequencing data: NCBI Sequence Read Archive (SRA) under BioProject PRJNA1425762• Proteomics data: PRIDE repository with the identifier PXD066488 Repository contents 1. NMR_RawData_MetaData.zip Contains processed 1H NMR spectral data and associated metadata used for metabolomic analysis. 2. Plots_16S.zip R scripts and processed data (corresponding to Supplementary Table S2) used to generate microbiome analysis figures and statistical analysis. 3. Plots_NMR.zip R scripts and processed data (corresponding to Supplementary Table S4) used for statistical analysis and visualization of NMR metabolomics data. 4. Plots_Weight.zip Scripts and processed data (corresponding to Supplementary Table S1) used for statistical analysis and visualization of body and gut weight measurements. ContactFor questions regarding the data or analysis pipeline, please contact:Saetbyeol Lee ([e-mail])