Monocyte CD4 mRNA and Protein Expression Analysis

CD14 protein costaining with RNA flow cytometry. The purpose was confirm monocytes as CD4 mRNA high and CD4 protein low, relative to lymphocyte population.
Conclusion:
CD14+ population (monocytes) was confirmed as CD4 protein low and CD4 mRNA high by RNA flow cytometry with protein costaining. Results were further supported through imaging results.
Notes:
Figure 1A,B and supplemental-Simultaneous flow cytometric analysis of IFN-g and CD4 mRNA and protein expression kinetics in human peripheral blood mononuclear cells during activation Instrument was setup using CST with FACSDiva software.